anti h3k9me2 3 (Cell Signaling Technology Inc)
Structured Review
Anti H3k9me2 3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 83 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/histone+3/Di%2FTri-Methyl-Histone+H3+(Lys9)+Mouse+mAb/pmc12989073-223-44-47
Average 94 stars, based on 83 article reviews
Images
Related Articles
Incubation:Article Title: Caveolin-1 modulates Notch transcriptional activity during in vitro respiratory multiciliated cell maturation Article Snippet: For SDS-PAGE we have used 4–20% Mini-PROTEAN ® TGX gels (Bio-Rad, #4561094) which allows for the separation of a wider range of protein size. .. The primary antibodies used were anti-Notch1 (Cell Signalling, #3608, 1:1000), anti-Notch2 (Cell Signalling, #5732, 1:1000), anti-GAPDH (Cell Signalling, #2118, 1:2500) and Article Title: Intermittent Fasting Enhances Genome Integrity and Cytoprotective Pathways via ( BHB ) β‐Hydroxybutyrate Signaling and Chromatin Remodeling Article Snippet: .. The membranes were blocked in Tris‐buffered saline with Tween 20 (20 mM Tris, pH 7.4, 150 mM NaCl, and 0.05% Tween 20) + 5% bovine serum albumin (BSA) (Sigma Aldrich, cat# A3912‐100G) for 1 h at room temperature and incubated overnight with H3K9bhb (1:1000; PTM Biolab, Chicago, IL, cat# PTM‐1250, RRID: AB_3076399), H3K27ac (Cell Signaling, Danvers, MA, cat# 8173, RRID: AB_10949503), rabbit anti‐Hif1a (1:1000; Invitrogen, Carlsbad, CA, cat# PA1‐16601, RRID: AB_2117128), Anti‐DNA/RNA Damage antibody (8‐oxo‐dG) (1:1000; Sigma‐Aldrich, St. Louis, MO, cat# SAB5200010‐100UG, RRID: AB_3713037), H3K27bhb (1:500; PTM Biolab, Chicago, IL, cat# PTM‐1293, RRID: AB_2927632), GAPDH Antibody (Santa Cruz, Dallas, TX, cat#: sc‐25 778, RRID: AB_10167668), and Article Title: Hippocampal Cdk5 is regulated by distinct stress paradigms in male and female mice Article Snippet: .. After blocking, membranes were incubated overnight at 4 °C with primary antibodies targeting Cdk5 (Cell Signaling #2506) and housekeeping controls (A-tubulin [Cell Signaling #2144], GAPDH [Cell Signaling #2118], B-actin [Santa Cruz #sc-517582] or Article Title: Caveolin-1 modulates Notch transcriptional activity during in vitro respiratory multiciliated cell maturation. Article Snippet: For SDS-PAGE we have used 4-20% Mini-PROTEAN® TGX gels (Bio-Rad, #4561094) which allows for the separation of a wider range of protein size. .. The primary antibodies used were anti-Notch1 (Cell Signalling, #3608, 1:1000), anti-Notch2 (Cell Signalling, #5732, 1:1000), anti-GAPDH (Cell Signalling, #2118, 1:2500) and other:Article Title: Synthesis of small molecule histone deacetylase 6 degraders, compounds formed thereby, and pharmaceutical compositions containing them Article Snippet: Antibodies against HDACs, IKZF1, IKZF3, Ac-α-Tubulin (K40), Histone-3, Ac-Histone-3 (K9), Caspase-3, PARP and anti-mouse- and Saline:Article Title: Intermittent Fasting Enhances Genome Integrity and Cytoprotective Pathways via ( BHB ) β‐Hydroxybutyrate Signaling and Chromatin Remodeling Article Snippet: .. The membranes were blocked in Tris‐buffered saline with Tween 20 (20 mM Tris, pH 7.4, 150 mM NaCl, and 0.05% Tween 20) + 5% bovine serum albumin (BSA) (Sigma Aldrich, cat# A3912‐100G) for 1 h at room temperature and incubated overnight with H3K9bhb (1:1000; PTM Biolab, Chicago, IL, cat# PTM‐1250, RRID: AB_3076399), H3K27ac (Cell Signaling, Danvers, MA, cat# 8173, RRID: AB_10949503), rabbit anti‐Hif1a (1:1000; Invitrogen, Carlsbad, CA, cat# PA1‐16601, RRID: AB_2117128), Anti‐DNA/RNA Damage antibody (8‐oxo‐dG) (1:1000; Sigma‐Aldrich, St. Louis, MO, cat# SAB5200010‐100UG, RRID: AB_3713037), H3K27bhb (1:500; PTM Biolab, Chicago, IL, cat# PTM‐1293, RRID: AB_2927632), GAPDH Antibody (Santa Cruz, Dallas, TX, cat#: sc‐25 778, RRID: AB_10167668), and Western Blot:Article Title: Compositions and methods for treating macrophage activation syndrome Article Snippet: The protein content in the lysates was measured by a 96-well plate-based Bradford assay (Cat no. 5000006, Biorad protein assay reagent concentrate). .. Western Blot Protein samples (10-12 μg) were resolved by SDS-PAGE on 4-12% Bis-Tris gels (Invitrogen), electrotransferred to nitrocellulose membranes (Cat no. 10484060, Bio-Rad (Hercules, CA)), and assessed for the immunosignal of proteins using the antibodies against HO-1 (cat no. ADI-SPA-896, Enzo (Farmingdale, NY)), Nrf2 (Cat no. 12721, Cell Signaling (Danvers, MA)), Erk (Cat no. 4695, Cell signaling), pErk (Cat no. 4379, Cell Signaling), Cyclophilin B (CyPB, Cat no. PA1-027A, ThermoFisher Scientific), β tubulin (Cat no. 2146, Cell Signaling), β actin (Cat No. 66009-1-Ig, Proteintech (Rosemont, IL)), Calnexin (Cat no. ab22595, abcam (Cambridge, UK) and SDS Page:Article Title: Compositions and methods for treating macrophage activation syndrome Article Snippet: The protein content in the lysates was measured by a 96-well plate-based Bradford assay (Cat no. 5000006, Biorad protein assay reagent concentrate). .. Western Blot Protein samples (10-12 μg) were resolved by SDS-PAGE on 4-12% Bis-Tris gels (Invitrogen), electrotransferred to nitrocellulose membranes (Cat no. 10484060, Bio-Rad (Hercules, CA)), and assessed for the immunosignal of proteins using the antibodies against HO-1 (cat no. ADI-SPA-896, Enzo (Farmingdale, NY)), Nrf2 (Cat no. 12721, Cell Signaling (Danvers, MA)), Erk (Cat no. 4695, Cell signaling), pErk (Cat no. 4379, Cell Signaling), Cyclophilin B (CyPB, Cat no. PA1-027A, ThermoFisher Scientific), β tubulin (Cat no. 2146, Cell Signaling), β actin (Cat No. 66009-1-Ig, Proteintech (Rosemont, IL)), Calnexin (Cat no. ab22595, abcam (Cambridge, UK) and Blocking Assay:Article Title: Hippocampal Cdk5 is regulated by distinct stress paradigms in male and female mice Article Snippet: .. After blocking, membranes were incubated overnight at 4 °C with primary antibodies targeting Cdk5 (Cell Signaling #2506) and housekeeping controls (A-tubulin [Cell Signaling #2144], GAPDH [Cell Signaling #2118], B-actin [Santa Cruz #sc-517582] or |
