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anti h3k9me2 3  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti h3k9me2 3
    Anti H3k9me2 3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 83 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/histone+3/Di%2FTri-Methyl-Histone+H3+(Lys9)+Mouse+mAb/pmc12989073-223-44-47
    Average 94 stars, based on 83 article reviews
    anti h3k9me2 3 - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Incubation:

    Article Title: Caveolin-1 modulates Notch transcriptional activity during in vitro respiratory multiciliated cell maturation
    Article Snippet: For SDS-PAGE we have used 4–20% Mini-PROTEAN ® TGX gels (Bio-Rad, #4561094) which allows for the separation of a wider range of protein size. .. The primary antibodies used were anti-Notch1 (Cell Signalling, #3608, 1:1000), anti-Notch2 (Cell Signalling, #5732, 1:1000), anti-GAPDH (Cell Signalling, #2118, 1:2500) and Histone 3 (Cell Signalling, #4499, 1:10000) incubated 1 h at room temperature. .. The HRP-conjugated secondary antibodies that were used are anti-Rabbit-HRP (ThermoFisher, #31460, 1:10000) and anti-Mouse-HRP (ThermoFisher, #62–6520, 1:5000) incubated 1 h at room temperature.

    Article Title: Intermittent Fasting Enhances Genome Integrity and Cytoprotective Pathways via ( BHB ) β‐Hydroxybutyrate Signaling and Chromatin Remodeling
    Article Snippet: .. The membranes were blocked in Tris‐buffered saline with Tween 20 (20 mM Tris, pH 7.4, 150 mM NaCl, and 0.05% Tween 20) + 5% bovine serum albumin (BSA) (Sigma Aldrich, cat# A3912‐100G) for 1 h at room temperature and incubated overnight with H3K9bhb (1:1000; PTM Biolab, Chicago, IL, cat# PTM‐1250, RRID: AB_3076399), H3K27ac (Cell Signaling, Danvers, MA, cat# 8173, RRID: AB_10949503), rabbit anti‐Hif1a (1:1000; Invitrogen, Carlsbad, CA, cat# PA1‐16601, RRID: AB_2117128), Anti‐DNA/RNA Damage antibody (8‐oxo‐dG) (1:1000; Sigma‐Aldrich, St. Louis, MO, cat# SAB5200010‐100UG, RRID: AB_3713037), H3K27bhb (1:500; PTM Biolab, Chicago, IL, cat# PTM‐1293, RRID: AB_2927632), GAPDH Antibody (Santa Cruz, Dallas, TX, cat#: sc‐25 778, RRID: AB_10167668), and histone 3 (Cell Signaling, Danvers, MA, RRID: AB_331563) antibodies at 4°C. .. The membranes were washed and then incubated with HRP conjugated anti‐mouse and anti‐rabbit secondary antibodies (1:10000; GE Healthcare) at room temperature for 1 h. A chemiluminescent signal was detected using PierceTM ECL Western blotting Substrate (Pierce Biotechnology, Waltham, MS, cat# 32106) by the ChemiDocTM XRS + molecular imager (BIO‐RAD, Hercules, CA, USA), and densitometric analysis was performed using ImageJ open‐source software.

    Article Title: Hippocampal Cdk5 is regulated by distinct stress paradigms in male and female mice
    Article Snippet: .. After blocking, membranes were incubated overnight at 4 °C with primary antibodies targeting Cdk5 (Cell Signaling #2506) and housekeeping controls (A-tubulin [Cell Signaling #2144], GAPDH [Cell Signaling #2118], B-actin [Santa Cruz #sc-517582] or Histone 3 [Cell Signaling # 9715]). .. After washing, membranes were incubated for 1 hr at room temperature with Horseradish Peroxidase (HRP)-conjugated anti-rabbit (Cell Signaling #7074) or anti-mouse (Cell Signaling #7076) secondary antibodies.

    Article Title: Caveolin-1 modulates Notch transcriptional activity during in vitro respiratory multiciliated cell maturation.
    Article Snippet: For SDS-PAGE we have used 4-20% Mini-PROTEAN® TGX gels (Bio-Rad, #4561094) which allows for the separation of a wider range of protein size. .. The primary antibodies used were anti-Notch1 (Cell Signalling, #3608, 1:1000), anti-Notch2 (Cell Signalling, #5732, 1:1000), anti-GAPDH (Cell Signalling, #2118, 1:2500) and Histone 3 (Cell Signalling, #4499, 1:10000) incubated 1h at room temperature. .. The HRP-conjugated secondary antibodies that were used are anti-Rabbit-HRP (ThermoFisher, #31460, 1:10000) and anti-Mouse-HRP (ThermoFisher, #62-6520, 1:5000) incubated 1h at room temperature.

    other:

    Article Title: Synthesis of small molecule histone deacetylase 6 degraders, compounds formed thereby, and pharmaceutical compositions containing them
    Article Snippet: Antibodies against HDACs, IKZF1, IKZF3, Ac-α-Tubulin (K40), Histone-3, Ac-Histone-3 (K9), Caspase-3, PARP and anti-mouse- and anti-rabbit HRP-linked antibodies were purchased from Cell Signaling Technology (CST; Danvers, Massachusetts, USA).

    Saline:

    Article Title: Intermittent Fasting Enhances Genome Integrity and Cytoprotective Pathways via ( BHB ) β‐Hydroxybutyrate Signaling and Chromatin Remodeling
    Article Snippet: .. The membranes were blocked in Tris‐buffered saline with Tween 20 (20 mM Tris, pH 7.4, 150 mM NaCl, and 0.05% Tween 20) + 5% bovine serum albumin (BSA) (Sigma Aldrich, cat# A3912‐100G) for 1 h at room temperature and incubated overnight with H3K9bhb (1:1000; PTM Biolab, Chicago, IL, cat# PTM‐1250, RRID: AB_3076399), H3K27ac (Cell Signaling, Danvers, MA, cat# 8173, RRID: AB_10949503), rabbit anti‐Hif1a (1:1000; Invitrogen, Carlsbad, CA, cat# PA1‐16601, RRID: AB_2117128), Anti‐DNA/RNA Damage antibody (8‐oxo‐dG) (1:1000; Sigma‐Aldrich, St. Louis, MO, cat# SAB5200010‐100UG, RRID: AB_3713037), H3K27bhb (1:500; PTM Biolab, Chicago, IL, cat# PTM‐1293, RRID: AB_2927632), GAPDH Antibody (Santa Cruz, Dallas, TX, cat#: sc‐25 778, RRID: AB_10167668), and histone 3 (Cell Signaling, Danvers, MA, RRID: AB_331563) antibodies at 4°C. .. The membranes were washed and then incubated with HRP conjugated anti‐mouse and anti‐rabbit secondary antibodies (1:10000; GE Healthcare) at room temperature for 1 h. A chemiluminescent signal was detected using PierceTM ECL Western blotting Substrate (Pierce Biotechnology, Waltham, MS, cat# 32106) by the ChemiDocTM XRS + molecular imager (BIO‐RAD, Hercules, CA, USA), and densitometric analysis was performed using ImageJ open‐source software.

    Western Blot:

    Article Title: Compositions and methods for treating macrophage activation syndrome
    Article Snippet: The protein content in the lysates was measured by a 96-well plate-based Bradford assay (Cat no. 5000006, Biorad protein assay reagent concentrate). .. Western Blot Protein samples (10-12 μg) were resolved by SDS-PAGE on 4-12% Bis-Tris gels (Invitrogen), electrotransferred to nitrocellulose membranes (Cat no. 10484060, Bio-Rad (Hercules, CA)), and assessed for the immunosignal of proteins using the antibodies against HO-1 (cat no. ADI-SPA-896, Enzo (Farmingdale, NY)), Nrf2 (Cat no. 12721, Cell Signaling (Danvers, MA)), Erk (Cat no. 4695, Cell signaling), pErk (Cat no. 4379, Cell Signaling), Cyclophilin B (CyPB, Cat no. PA1-027A, ThermoFisher Scientific), β tubulin (Cat no. 2146, Cell Signaling), β actin (Cat No. 66009-1-Ig, Proteintech (Rosemont, IL)), Calnexin (Cat no. ab22595, abcam (Cambridge, UK) and Histone 3 (Cat no. 4499, Cell Signaling). .. Fluorescence tagged respective secondary antibodies obtained from Li-COR (Lincoln, Neb.)—Goat-anti-Rabbit-antibody (IRDye® 800CW), or Donkey-anti-mouse-antibody (IRDye® 800CW) or Donkey anti-Mouse IgG (IRDye® 680RD) were then used.

    SDS Page:

    Article Title: Compositions and methods for treating macrophage activation syndrome
    Article Snippet: The protein content in the lysates was measured by a 96-well plate-based Bradford assay (Cat no. 5000006, Biorad protein assay reagent concentrate). .. Western Blot Protein samples (10-12 μg) were resolved by SDS-PAGE on 4-12% Bis-Tris gels (Invitrogen), electrotransferred to nitrocellulose membranes (Cat no. 10484060, Bio-Rad (Hercules, CA)), and assessed for the immunosignal of proteins using the antibodies against HO-1 (cat no. ADI-SPA-896, Enzo (Farmingdale, NY)), Nrf2 (Cat no. 12721, Cell Signaling (Danvers, MA)), Erk (Cat no. 4695, Cell signaling), pErk (Cat no. 4379, Cell Signaling), Cyclophilin B (CyPB, Cat no. PA1-027A, ThermoFisher Scientific), β tubulin (Cat no. 2146, Cell Signaling), β actin (Cat No. 66009-1-Ig, Proteintech (Rosemont, IL)), Calnexin (Cat no. ab22595, abcam (Cambridge, UK) and Histone 3 (Cat no. 4499, Cell Signaling). .. Fluorescence tagged respective secondary antibodies obtained from Li-COR (Lincoln, Neb.)—Goat-anti-Rabbit-antibody (IRDye® 800CW), or Donkey-anti-mouse-antibody (IRDye® 800CW) or Donkey anti-Mouse IgG (IRDye® 680RD) were then used.

    Blocking Assay:

    Article Title: Hippocampal Cdk5 is regulated by distinct stress paradigms in male and female mice
    Article Snippet: .. After blocking, membranes were incubated overnight at 4 °C with primary antibodies targeting Cdk5 (Cell Signaling #2506) and housekeeping controls (A-tubulin [Cell Signaling #2144], GAPDH [Cell Signaling #2118], B-actin [Santa Cruz #sc-517582] or Histone 3 [Cell Signaling # 9715]). .. After washing, membranes were incubated for 1 hr at room temperature with Horseradish Peroxidase (HRP)-conjugated anti-rabbit (Cell Signaling #7074) or anti-mouse (Cell Signaling #7076) secondary antibodies.



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    Mice bearing late-stage Med-411FH tumors exhibiting LM were treated with empty (non-drug loaded) bCDN 5 or Panobinostat-loaded pCDN 5 nanoparticles. Tumors were harvested and analyzed 6 hours after treatment. (A) Western blot showing <t>H3K9ac</t> protein levels in cerebellum tumor cells treated with bCDN 5 or pCDN 5 nanoparticles. (B) Quantification of relative H3K9ac normalized to B-ACTIN from subpanel (A). (C, D) qRT-PCR showing FOXO1 mRNA quantification relative to B-ACTIN in tumor cells extracted from the cerebellum (C) or spine metastasis (D). Data in (B-D) represent the mean value of 6 mice per group, with error bars representing the standard error of the mean. P-values are reported on the following scale: *<0.05, **<0.01, ***<0.001, ****<0.0001.
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    Image Search Results


    Mice bearing late-stage Med-411FH tumors exhibiting LM were treated with empty (non-drug loaded) bCDN 5 or Panobinostat-loaded pCDN 5 nanoparticles. Tumors were harvested and analyzed 6 hours after treatment. (A) Western blot showing H3K9ac protein levels in cerebellum tumor cells treated with bCDN 5 or pCDN 5 nanoparticles. (B) Quantification of relative H3K9ac normalized to B-ACTIN from subpanel (A). (C, D) qRT-PCR showing FOXO1 mRNA quantification relative to B-ACTIN in tumor cells extracted from the cerebellum (C) or spine metastasis (D). Data in (B-D) represent the mean value of 6 mice per group, with error bars representing the standard error of the mean. P-values are reported on the following scale: *<0.05, **<0.01, ***<0.001, ****<0.0001.

    Journal: bioRxiv

    Article Title: Nanoparticle encapsulation enhances spatial distribution of Panobinostat to treat metastatic medulloblastoma via the intrathecal route

    doi: 10.64898/2026.03.31.715392

    Figure Lengend Snippet: Mice bearing late-stage Med-411FH tumors exhibiting LM were treated with empty (non-drug loaded) bCDN 5 or Panobinostat-loaded pCDN 5 nanoparticles. Tumors were harvested and analyzed 6 hours after treatment. (A) Western blot showing H3K9ac protein levels in cerebellum tumor cells treated with bCDN 5 or pCDN 5 nanoparticles. (B) Quantification of relative H3K9ac normalized to B-ACTIN from subpanel (A). (C, D) qRT-PCR showing FOXO1 mRNA quantification relative to B-ACTIN in tumor cells extracted from the cerebellum (C) or spine metastasis (D). Data in (B-D) represent the mean value of 6 mice per group, with error bars representing the standard error of the mean. P-values are reported on the following scale: *<0.05, **<0.01, ***<0.001, ****<0.0001.

    Article Snippet: 40μg of protein per lane was probed for histone 3 lysine-9 acetylation (H3K9ac) using a 1:1000 dilution of primary antibody (Cell Signaling Technology #9649) followed by a 1:1000 dilution of HRP-conjugated anti-rabbit secondary antibody (Cell Signaling Technology #7074), and detected by chemiluminescence (Clarity Western ECL Substrate, BIO-RAD #1705060).

    Techniques: Western Blot, Quantitative RT-PCR